Replication competition drives the selective mtDNA inheritance in Drosophila ovary

The data file contains 10 raw data folders, with microscopy images, excel files for quantifications, compressed 3D microscopy images, and two movies.
Figure 1. CAR assay effectively detects mitochondrial nucleoids in the Drosophila germarium.
Figure 2. Specificity and accuracy of the CAR assay in distinguishing mt:ND2ins and mt:CoIT300I mtDNA variants in situ.
Figure 3. Germline selection against the mt:CoIT300I genome occurs at germarium region 2B.
Figure 4. The mt:ND2ins genome replicates more efficiently than the deleterious mt:CoIT300I genome at germarium region 2B.
Supplemental Figure S1. The CasPLA method cannot spatially distinguish mtDNA variants in Drosophila ovaries (related to Figure 1).
Supplemental Figure S2. Applying both probes together in homoplasmic- or heteroplasmic ovaries using CAR assay (related to Figure 2).
Supplemental Figure S3. The mt:ND2ins genome replicates more efficiently and thereby outcompetes mt:CoIT300I genome at germarium region 2B (related to Figure 3 and Figure 4).
Supplemental Figure S4. Temperature dependent increase of mtDNA nucleoids in late germarium stages (related to Figure 3).
Movie-1. 3D rendering of CAR images and germarium cysts from heteroplasmic flies maintained at 18°C (related to Figure 3). The raw 3D image can be found in the file named Figure 3C original image.tif.zip, located in the Figure 3C folder.
Movie-2. 3D rendering of CAR images and germarium cysts from heteroplasmic flies maintained at 29°C (related to Figure 3). The raw 3D image can be found in the file named Figure 3D original image.tif.zip, located in the Figure 3D folder.