Investigation of the gamma-tubulin binding site in the nuclear and mitochondrial DNA of cells in S-phase

In a cell, g-tubulin establishes a cellular network of threads named g-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that g-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that g-tubulin has a role in the maintenance of the mitochondrial meshwork and homeostasis as reduced levels of g-tubulin or impairment of its GTPase domain disrupts the mitochondria network and alters both their respiratory capacity and the expression of mitochondria-related genes. By contrast, reduced mitochondria number or increased protein levels of g-tubulin DNA-binding domain favour the association of g-tubulin with mitochondria. Our results demonstrate that g-tubulin is an important mitochondrial structural component that maintains the mitochondria meshwork, providing mitochondria with a cellular infrastructure. We propose that g-tubulin provides a cytoskeletal element that maintains mitochondria homeostasis and gives form to the mitochondria meshwork. Keywords: Genome binding/occupancy profiling by high throughput sequencing The experiment was performed in an MCF10A cell line and a total number of 16 samples were included. There are two samples per timepoint (one and two hours) and a whole cell lysate (WCE) is included as a control for each sample. Please note that processed data was generated from two samples (replicates *.1 and *.2) and is linked to the first replicate (i.e. *1.1, *2.1) of each pair.