Histone Acetylation Regulator Gcn5 Mediates Drug Resistance and Virulence of Candida glabrata

This study explored the transcriptional change of Candida glabrata cells due to GCN5 deletion. We also compared the transcriptional responses to fluconazole and micafungin in WT and gcndelta cells relative to respective no drug controls. Overall design: Overnight culture of WT and gcn5? was inoculated in liquid YPD at an initial OD600 of 0.2 and grown at 37°C with shaking to OD600 of 0.6. Cells were then treated with fluconazole (8µg/ml) or micafungin (15ng/ml) for 2 hours. Untreated WT and gcn5? cells were grown in parallel. Cells were harvested at the end of 2h treatment and subjected to RNA extraction with two biological replicates for each strain/treatment group.