Raw 16S rRNA Illumina FASTQ Data from a Golden Lion Tamarin Microbiota Study on the Effects of Higher Dietary Fibre

This dataset contains raw paired-end 16S rRNA gene sequencing data (FASTQ files) generated from a study investigating the impact of increased dietary fibre on the faecal microbiome of golden lion tamarins (Leontopithecus rosalia) housed at Adelaide Zoo, South Australia. The research assessed microbial changes before and after a higher dietary fibre intervention. DNA was extracted using the QIAamp DNA Stool Kit (Qiagen). The sequencing was performed on the Illumina MiSeq platform, targeting the V3–V4 region of the bacterial 16S rRNA gene (2 × 300 bp, V3 chemistry). Sequencing carried out at the South Australian Genomics Centre, South Australian Health & Medical Research Institute (SAHMRI). These data support the manuscript: Higher dietary fibre increases the faecal microbiome diversity of golden lion tamarins (Leontopithecus rosalia), submitted to Animals (MDPI), manuscript ID: animals-3685841. This dataset was used for microbiota composition analysis, taxonomic profiling, and uncovering the microbial community shifts in response to higher fibre dietary intervention in golden lion tamarins (Leontopithecus rosalia). The dataset includes: 90 paired-end FASTQ files (45 samples × 2 reads) from golden lion tamarins collected with and without the high fibre dietary intervention 2 paired-end FASTQ files from a negative (blank) control sample (1 water control × 2 reads) used to monitor contamination during sequencing Metadata file (CSV) containing sample ID, animal ID, diet group (high fibre on/off), and sex