ChIP-seq and RNA-seq analysis reveals the association between thyroid hormone receptor a and skeletal muscle aging in mice(ChIP-seq)

In the current situation of the aging of the global population, primary sarcopenia is gradually attracting attention because of its potential risk to the elderly, high occult incidence and serious prognosis. As one of the most important hormones affecting skeletal muscle, the aging changes of thyroid hormone have been proved to be closely related to sarcopenia. Thyroid hormone exerts its effects locally in skeletal muscle by binding to thyroid hormone receptor. TRa is the main distribution type of thyroid hormone receptor in skeletal muscle. We have previously found the potential role of TRa in the aging process of skeletal muscle. As a nuclear transcription factor, TRa exerts subsequent biological effects by targeting regulatory genes. However, in the process of skeletal muscle aging, the target regulation map of TRa is still unclear.In this study, we constructed natural aging mouse models of different ages, combined ChIP-seq and mRNA-seq to explore the aging changes of TRa targets in skeletal muscle. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChlP-seq) for thyroid hormone receptor a, TRa in gastrocnemius muscle tissues of 6, 15, and 21 month old mice. Two mice from each age group were selected for analysis.