Protein Amount in Conditioned Medium of MenSCs

Protein microarray containing 1000 factors was used to detect protein components in the conditioned media of MenSCs. Select the protein with high expression content, and the enrichment result shows that it is related to inflammatory response, negative regulation of apoptotic process, microglial cell activation, negative regulation of TNF-α production, and innate immune response. These biological processes may partly explain the therapeutic functions of MenSCs on promoting the survival of DAergic neurons and anti-inflammation by regulating microglia polarization. Protein array kit (GSH-CAA-X00-1, RayBiotech Life Inc., Norcross, GA, USA) was used to detect the amount of 1000 kinds of factors in the conditioned medium of MenSCs (MenSCs-CM).Two samples were included. The preparation method of MenSCs is as follows. When MenSCs were 70%–80% sub-confluent, the growth medium was replaced with 20 mL serum-free DMEM-HG medium (containing 100 m/mL penicillin, 100 mg/mL streptomycin). Culture medium was collected after 48 h incubation, centrifuged at 600 g for 5 min and passed through a 0.22 mm filter, which was termed as MenSCs-CM. Signals were scanned by InnoScan 300 Microarray Scanner. Select the protein with high expression content, and the enrichment result shows that it is related to inflammatory response, negative regulation of apoptotic process, microglial cell activation, negative regulation of TNF-α production, and innate immune response. These biological processes may partly explain the therapeutic functions of MenSCs on promoting the survival of DAergic neurons and anti-inflammation by regulating microglia polarization.