Next generation sequencing of chicken otocyst with gain- and loss-of-Notch signalling activity. Next generation sequencing of chicken otocyst with gain- and loss-of-Notch signalling activity

Bulk RNA-sequencing of individual chicken otocysts, in which Notch activity was either stimulated or blocked. To stimulate Notch activity, the E2 otic placodes were in ovo transfected with a construct overexpressing Notch1 Intracellular domain (NICD1) or a control plasmid expressing mRFP1 and collected 6h or 24h later. To pharmacologically block Notch activity, E2.5 chicken otocysts were incubated in media enriched with γ-secretase Inhibitor or control condition (DMSO) for 6h or 24h. Transcripts abundance was mapped to a chicken reference genome using Kallisto package. Overall design: Identification of genes deregulated in the otocyst in response to the manipulation of Notch signaling activity