Homo sapiens fecal microbiome transplant

The objective of the study was to investigate the efficacy of fresh, frozen or lyophilized fecal microbiota transplantation (FMT) via colonoscopy in patients with recurrent C. difficile associated diarrhea (RCDAD). After a course of oral vancomycin we performed FMT in 36 patients with =3 bouts of RCDAD. FMT products from thoroughly screened healthy donors after filtering stools twice were given as single (non-pooled) product either as fresh, frozen (-80C) or lyophilized. Patients submitted fresh stools pre- and on 7, 14 and 30 days post-FMT. Bacterial DNA was extracted and the V4 region of the 16S rRNA gene was amplified by PCR and amplicons were sequenced on the MiSeq (Illumina) platform. The unique aspects of this study are the comparative efficacy in different patients with recurrent CDI of FMT using the same donor providing either fresh or frozen product, or the successful use of lyophilized FMT products.