Concentration-dependent effect of delta-9-tetrahydrocannabinol on epigenetic DNA modifiers in human peripheral blood mononuclear cells

Genome-wide patterns of DNA methylation were measured using Reduced Representation Bisulfite Sequencing (RRBS) and Reduced Representation Oxidative Bisulfite Sequencing (RRoxBS). Human peripheral blood mononuclear cells were isolated from the whole blood of healthy male volunteers. We used an in vitro study design, which consisted of repeated THC incubations followed by an "abstinence" period and a THC challenge incubation. 24 hours after the THC challenge incubation, cells were collected, DNA was extracted, and sent to sequencing.