Transcriptome analysis of auditory cortex in 1-month-old and 1-year-old Ash1l heterozygous mice with their age-matched WT littermates

Ash1l encodes a histone methyltransferase, a member of the trithorax group proteins, which regulates developmental essential gene expression by catalyzing H3K36 methylation and counteracting polycomb silencing. Accumulating reports suggest the loss-of-function mutants in Ash1l gene are associated with intellectual disability (ID), attention-deficit/hyperactivity (ADHD), autism spectrum disorder (ASD), Tourette syndrome (TS) and multiple congenital anomalies (MCA). We performed transcriptome analysis of auditory cortex in 1-month-old and 1-year-old Ash1l Ash1l heterozygous mice with their age-matched WT littermates via RNA sequencing (RNA-seq). Ash1l haploinsufficiency induces transcription alternation of genes involved in synaptic function and cortical development, implicating the deficits in synapse pruning and behavior in adult mice. 3 WT (1-month-old) and 3 Ash1l heterozygous (1-month-old) auditory cortex;2 WT (1-year-old) and 2 Ash1l heterozygous (1-year-old) auditory cortex