Breast Cancer Deubiquitinating Enzyme (DUB) Inhibitor Profiling: Fixed-cell GR measures of 21 breast cell lines to 32 small molecule perturbagens. Dataset 1 of 2: Normalized growth rate inhibition values.

We measured the sensitivities of one non-malignant breast cell line and 20 breast cancer cell lines (six triple negative, six hormone receptor positive, five Her2 amplified, and three established from triple negative patient-derived xenografts) to 32 pre-clinical small molecule deubiquitinating enzyme (DUB) inhibitors. A microscopy-based dose response assay was used to measure drug potency, and to quantify drug efficacy in terms of growth inhibition (GR metrics) and cell death. We treated cells with single drugs over a 9-point ½ log dilution series and then measured cell number and viability after three days of drug exposure.