Mutation breeding of hypoxia tolerance and super growth Megalobrama amblycephala by a novel mutagenesis with atmospheric and room temperature plasma

We used a novel atmospheric and room temperature plasma (ARTP) mutagenesis tool to successfully mutagen blunt-snout bream (Megalobrama amblycephala) for the first time and obtain HS (Hypoxia tolerance and Super growth) mutants of blunt-snout bream. A total of 93.2GB clean data were received from two control groups (3 fish in each group) and two HS groups (3 fish in each group) randomly selected, including 3132418 to 3223481SNPs and 912886 to 934664 InDels. The average mutation rate at the genome level is about 0.30%. 3651 HS group special non-synonymous SNP mutations are located in 1223 genes. Combining kegg and go terms, we screened out 4 hypoxia-related (Epo X1, VEGFR1, HO-1a, LPAR6) genes and 5 growth-related genes (FAS, 6-PFK, somatostatin-1A, zinc transporter ZIP4, Apolipoprotein Eb), which were non-synonymous mutations at one or more sites, and there is also a homozygous HO-1a. The result of these revelation will be useful in future selections of genetic breeding for hypoxia-tolerance and growth traits in blunt-snout bream.