(Epi)genetic regulation of zebrafish intestinal development

The goal of the experiment was to characterize the wild type larval intestine in zebrafish. Tg(cldn15la:GFP) intestine-specific transgene was used to ease dissections. RNAseq was performed at 5, 7, 9 dpf on wild type dissected intestines to characterize the transcriptome. ChIPseq was performed at 5, 7, 9 dpf on wild type dissected intestines to check the intestinal presence of active (H3K4me3) and repressive (H3K27me3) chromatin marks and to correlate their presence with intestinal gene expression. RNAseq of wild type dissected Danio rerio intestines, pools of 10 intestines in triplicates at 5, 7, and 9 dpf. ChIPseq of wild type dissected Danio rerio intestines, pools of 25 intesines (+5 set aside for input DNA) in duplicates at 5, 7, and 9 dpf for H3K4me3 and H3K27me3.