Development of gene expression signatures for derivatives of mangiferin

To further development of our gene expression approach to pharmacology, we have employed whole genome microarray expression profiling as a discovery platform to identify gene expressionsignatures for 2 derivatives of mangiferin. 3T3-L1 preadipocytes were incubated with X-1 or X-3 for 24 h, and a 19-gene consensus signature was identified that distinguished between treatment and control samples. X-1 or X-3 induced gene expression in 3T3-L1 cells was measured at 24 hours after exposure to 70 µM. Nine independent experiments were performed at 24 hours.