Differential effects of tree species identity on rhizospheric bacterial and fungal community richness and composition across multiple trace element-contaminated sites

Soil microbial communities play a key role in plant nutrition and stress tolerance. This is particularly true in sites contaminated by trace metals, which often have low fertility and stressful conditions for woody plants in particular. However, we have limited knowledge of the abiotic and biotic factors affecting the richness and composition of microbial communities inhabiting the rhizosphere of plants in contaminated sites. Using high-throughput amplicon sequencing, we studied the rhizospheric bacterial and fungal community structures of 14 woody plant families planted in three contrasting sites contaminated by metals (Pb, Cd, Zn, Mn, Fe, S). The rhizospheric bacterial communities in the given sites showed no significant difference between the various woody species but did differ significantly between sites. The Proteobacteria phylum was dominant, with over 25% of the overall relative abundance, followed by Actinobacteria, Bacteroidetes, and Gemmatimonadetes. Sites were also the main ..., , , # Differential effects of tree species identity on rhizospheric bacterial and fungal community richness and composition across multiple trace element-contaminated sites

[https://doi.org/10.5061/dryad.prr4xgxsp](https://doi.org/10.5061/dryad.prr4xgxsp)

This Readme file lists the raw sequencing fastq files obtained for the Bact02 and Fung02 markers, explains how they were obtained and the structure of the descriptor files that can be used to assign each sequence read to the right PCR replicate. Finally, the last section explains the structure of the

## Description of the data and file structure

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### Bact02 marker

After PCR amplification, amplicons were pooled in equal volumes in two separate batches A and B. Each batch was used to prepare a sequencing library.

Library GWM-1320 corresponds to batch A, and library GWM-1321 corresponds to batch B. Each library was sequenced on two different Illumina HiSeq2500 sequencing lanes.

Ultimately, this resulted in 4 R1 fastq sequence files...