Expression data from Saccharomyces cerevisiae

Reprogramming a non-methylotrophic industrial host, such as Saccharomyces cerevisiae, to a synthetic methylotroph reprents a huge challenge due to the complex regulation in yeast. Through TMC strategy together with ALE strategy, we completed a strict synthetic methylotrophic yeast that could use methanol as the sole carbon source. However, how cells respond to methanol and remodel cellular metabolic network on methanol were not clear. Therefore, genome-scale transcriptional analysis was performed to unravel the cellular reprograming mechanisms underlying the improved growth phenotype. Overall design: To understand how cells reprogram cellular metabolism to adapt growth on methanol,the evolved strain CX01F (B1-3) was compared with its parental strain CX01 (Sample1-3).