Metagenomic characterization of rhizosphere microbial communities in forest ecosystems invaded by Impatiens parviflora.

Metagenomic sequencing was conducted to characterize rhizosphere-associated microbial communities in forest ecosystems invaded by Impatiens parviflora in northern Poland. Soil samples were collected from multiple plots in a mixed forest habitat within the Kudypy Forest District (Olsztyn, Poland). Whole-metagenome shotgun libraries were prepared from extracted soil DNA and sequenced using the Oxford Nanopore MinION platform. The resulting long-read metagenomic datasets were used to evaluate the taxonomic composition and functional potential of soil microbiomes associated with the I. parviflora rhizosphere and to compare them with microbiomes linked to native plant communities. These data provide a valuable resource for exploring plant–soil–microbe interactions and microbial traits that may contribute to the invasion success of I. parviflora in temperate European forests.