Nature Methods - ResearchHelpDesk

Nature Methods - ResearchHelpDesk - Nature Methods is a monthly journal publishing novel methods and significant improvements to basic life sciences research techniques. All editorial decisions are made by a team of full-time professional editors. Nature Methods is a forum for the publication of novel methods and significant improvements to tried-and-tested basic research techniques in the life sciences. This monthly publication is aimed at a broad, interdisciplinary audience of academic and industry researchers actively involved in laboratory practice. It provides them with new tools to conduct their research and places a strong emphasis on the immediate practical relevance of the work presented. The journal publishes primary research papers as well as overviews of recent technical and methodological developments. We are actively seeking primary methods papers of relevance to the biological and biomedical sciences, including methods grounded in chemistry that have a practical application to the study of biological problems. To enhance the practical relevance of each paper, description of the method must be accompanied by its validation, its application to an important biological question and results illustrating its performance in comparison to available approaches. Articles are selected for publication that present broad interest, thorough assessments of methodological performance and comprehensive technical descriptions that facilitate immediate application. Specific areas of interest include, but are not limited to: Methods for nucleic acid manipulation, amplification and sequencing Methods for protein engineering, expression and purification Methods for proteomics, including mass spectrometry, analysis of binding interactions, microarray-based technologies, display techniques, analysis of post-translational modifications, glycobiology and metabolomics Methods for systems biology, including proteomics approaches, protein interaction analysis methods and genome wide expression and regulation profiling Biomolecular structural analysis technologies, including NMR and crystallography Chemical biology techniques, including chemical labeling, methods for expanding the genetic code and directed evolution Biophysical methods, including single molecule and lab-on-a-chip technologies Optical and non-optical imaging technologies, including probe design and labeling methods, microscopy, spectroscopy and in vivo imaging Techniques for the analysis and manipulation of gene expression, including epigenetics, gene targeting, transduction, RNA interference and microarray-based technologies Methods for cell culture and manipulation, including stem cells, single cell methods and lab-on-a-chip technologies Immunological techniques, including production of antibodies, antibody-based assays and immunolabeling Methods for the study of physiology and disease processes including cancer Methods involving model organisms and their manipulation and phenotyping Computational and bioinformatic methods for analysis, modeling or visualization of biological data Nanotechnology-based methods applied to basic biology

《自然方法》杂志每月出版新颖的方法和基本生命科学研究技术的显著改进。所有编审决策均由一支全职专业编辑团队作出。《自然方法》杂志是发布新颖方法和对经过验证的基本研究技术的显著改进的论坛。该月度出版物面向广泛、跨学科的学术和产业研究人员，他们积极参与实验室实践。它为他们提供新的研究工具，并强调所呈现工作的即时实际相关性。该杂志发表原创研究论文以及近期技术和方法论发展的综述。我们积极寻求与生物和生物医学科学相关的原创方法论文，包括基于化学的方法，这些方法在生物问题研究中具有实际应用。为了增强每篇论文的实际相关性，必须提供方法描述的验证、将其应用于重要的生物学问题以及与现有方法相比的性能结果。选定的文章将具有广泛的兴趣、详尽的方法论性能评估和促进即时应用的全面技术描述。具体感兴趣领域包括但不限于：核酸操作、扩增和测序方法，蛋白质工程、表达和纯化方法，蛋白质组学方法，包括质谱、结合相互作用分析、基于微阵列的技术、展示技术、翻译后修饰分析、糖生物学和代谢组学方法，系统生物学方法，包括蛋白质组学方法、蛋白质相互作用分析方法以及全基因组表达和调控分析，生物分子结构分析方法，包括核磁共振和晶体学，化学生物学技术，包括化学标记、扩展遗传编码的方法和定向进化，生物物理方法，包括单分子和芯片实验室技术，光学和非光学成像技术，包括探针设计和标记方法、显微镜、光谱学和体内成像，基因表达分析和操作技术，包括表观遗传学、基因靶向、转导、RNA干扰和基于微阵列的技术，细胞培养和操作方法，包括干细胞、单细胞方法和芯片实验室技术，免疫学技术，包括抗体的生产、基于抗体的检测和免疫标记，研究生理和疾病过程的方法，包括癌症，涉及模式生物及其操作和表型分析，分析和可视化生物数据的计算和生物信息学方法，应用于基础生物学的基于纳米技术的方法。