RNA-expression profiling of mouse B cells coexpressing LMP1 with EBNA3A, Rel or Ebf1 in vitro

mouse B cells coexpressing LMP1 with EBNA3A, Rel or Ebf1 were cultured for 6 days in vitro and analysed by RNA-sequencing Overall design: CD43- splenic B cells from R26LMP1stopf mice were isolated and treated with TAT-cre (iLMP1 B cells). After 24h cells were tranduced with MSCV (MIG)-vectors encoding indicated genes. At day 6 cells were sorted for MSCV-reporter (GFP) expression. Alternatively, CD43- splenic B cells from R26LMP1stopf and R26LMP1stopf/R26EBNA3Astopf mice were isolated, treated with TAT-cre (iLMP1 and iLMP1/EBNA3A B cells) and sorted at day 6. RNA from sorted cells was isolated and RNA-sequenced.