PATIENTS ANTIBODIES RECOGNIZED TWO ISOENZYMES OF GLYCERALDEHYDE -3P- DESHYDROGENASE OF ECHINOCOCCUS GRANULOSUS s. l. Cloning epitopes and enzymatic analysis in silico

Our group is working identifying antigens from E. granulosus s.l by immune-identification and LC-MS/MS methodologies, CE patient sera recognized antigens expressed by cell line from bovine’s protoscolices E .granulouss G1 (EGPE). The antigenic model from EGPE cells showed more sensitivity for patient’s sera reactivity than HF obtained for sheep’s E. granulosus G1 . EGPE cells were growth in liquid media and in biphasic agarose medium forming cysts colonies . This cellular model allows us to study expressed proteins mainly found intracellular (IC) or extracellular (EC) compartments.