ATAC-seq Identification of Chromatin Accessibility in Both Human Host Cells and the Parasite Toxoplasma gondii

To identify accessible chromatin regions in the human host cells during Toxoplasma parasite infection (uninfected, RH-infected and Pru-infected human foreskin fibroblasts) and in the obligate intracellular parasite Toxoplasma gondii (Type 1 RH strain and Type 2 Pru strain), ATAC-seq was performed. Four biological replicates of 1) uninfected human foreskin fibroblasts (HFFs), 2) T. gondii Type 1 RH-infected, and 3) T.gondii Type 2 Pru-infected HFFs at 24-hr post-infection.