The functional enhancer repertoire of human embryonic stem cells [plasmid DNA-seq]

We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the DNA-seq component from plasmid libraries prior to transfection. Overall design: For each ChIP-seq and BAC library: DNA-seq of ChIP-STARR-seq and BAC-STARR-seq plasmid library = 12 datasets