Secretomic analysis of cheap enzymatic cocktails of <i>Aspergillus niger</i> LBM 134 grown on cassava bagasse and sugarcane bagasse

Currently, agroindustrial wastes are little used for generating value-added products; hence, their use of these waste to produce enzymatic cocktails for the conversion of lignocellulosic biomass to fermentable sugars is a very interesting alternative in the second-generation bioethanol process. The Ascomycota fungus <i>Aspergillus niger</i> LBM 134 produces hydrolytic enzymes in large proportions. In this work, <i>A. niger</i> LBM 134 was grown on sugarcane and cassava bagasses under optimized conditions. To identify the extracellular enzymes involved in the degradation of these agroindustrial wastes, the secretomes of the culture supernatants of the fungus were analyzed and validated by biochemical assays of the enzymatic activities. <i>A. niger</i> LBM 134 secreted higher quantities of xylanases and accessory hemicellulases when it grew on sugarcane bagasse, whereas more cellulases, amylases, and pectinases were secreted when it grew on cassava bagasse. These findings suggest two promising enzyme cocktails for the hydrolysis of lignocellulose carbohydrate polymers to fermentable sugars. These bioinformatic analysis were functional validates through enzymatic biochemical assays that confirm the biotechnological potential of <i>A. niger</i> LBM 134 for the bioconversion of hemicellulosic substrates such as sugarcane and cassava bagasses.

当前，农业工业废弃物（agroindustrial wastes）在增值产物生产中的应用仍较为有限，因此利用此类废弃物制备酶复合体系（enzymatic cocktails），将木质纤维素生物质（lignocellulosic biomass）转化为可发酵糖，是第二代生物乙醇（second-generation bioethanol）生产工艺中极具潜力的替代方案。子囊菌门（Ascomycota）真菌黑曲霉（Aspergillus niger）LBM 134可大量分泌水解酶类。本研究中，我们在优化后的培养条件下，以甘蔗渣与木薯渣为底物培养黑曲霉LBM 134。为鉴定参与降解此类农业工业废弃物的胞外酶类，我们对该真菌的培养上清液分泌组（secretome）进行了分析，并通过酶活性生化实验完成验证。当以甘蔗渣为底物时，黑曲霉LBM 134可分泌更多的木聚糖酶（xylanases）与辅助性半纤维素酶（accessory hemicellulases）；而以木薯渣为底物时，其分泌的纤维素酶（cellulases）、淀粉酶（amylases）与果胶酶（pectinases）含量更高。上述研究结果表明，可获得两种极具应用前景的酶复合体系，用于将木质纤维素类碳水化合物聚合物水解为可发酵糖。本研究通过酶活性生化实验对生物信息学分析结果进行了功能验证，证实了黑曲霉LBM 134在将甘蔗渣、木薯渣等半纤维素底物进行生物转化方面的生物技术应用潜力。

搜集汇总

背景与挑战

背景概述

该数据集研究了真菌Aspergillus niger LBM 134在甘蔗渣和木薯渣上培养时分泌的酶类差异，发现甘蔗渣促进木聚糖酶分泌，而木薯渣促进纤维素酶、淀粉酶和果胶酶分泌。研究通过生物化学实验验证了这些酶类在木质纤维素转化中的潜力，为开发低成本酶制剂提供了数据支持。