m6A调控地中海涡虫再生

为了从我们自己的角度概述涡虫再生过程中的基因表达动态，我们在损伤后的五个不同时间点进行了RNA-seq（0小时，6小时，3天，7天，11天）。随后，对相同的五个时间点的wtap敲低及对照样本进行了MeRIP-seq测序，以了解涡虫再生中的m6A修饰基因及水平变化。最后，进行了wtap敲低前后的单细胞测序，以了解m6A缺失后详细的细胞类型变化。

To profile the dynamics of gene expression during planarian regeneration, we performed RNA-seq at five distinct post-injury time points: 0 h, 6 h, 3 d, 7 d, and 11 d. Subsequently, we conducted MeRIP-seq on both WTAP knockdown and control samples collected at the same five time points to investigate changes in m6A-modified genes and their modification levels during planarian regeneration. Finally, we performed single-cell RNA-seq before and after WTAP knockdown to characterize the detailed cell type alterations following m6A depletion.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于m6A（一种RNA修饰）在地中海涡虫再生过程中的调控作用，通过RNA-seq、MeRIP-seq和单细胞测序技术，在损伤后多个时间点（0小时至11天）分析基因表达和m6A修饰的动态变化，并探究WTAP敲低对细胞类型的影响。数据集包含70个样本，总计1.37 TB，旨在从组学角度揭示m6A在再生生物学中的关键机制。