Transcriptome Profiling of Japanese Encephalitis Virus (JEV) Infected Human Neuroepithelioma Cells (SK-N-MC)

Japanese Encephalitis (JE) is an important causal of viral encephalitis in Eastern and Southeast Asia. Neuronal injury and varying degrees of neuroinflammation are often pathological hallmarks of JE, which result in high morbility and mortality rates. However, a clear understanding on neuron cells' responses to JEV infection remains elusive. This study, therefore, utilized the Affymetrix Human PrimeView array for a robust profiling of JEV infected SK-N-MC cells during the active phase of infection in order to investigate the molecular details of the host cell responses. SK-N-MC cells were mock and JEV infected at Multiplicity of Infection (MOI) 10. Mock infections were achieved by subtitution of the viruses with equal amount of medium. Total RNA of mock and JEV infected cells were extracted at 48 and 60 hour post infection. Total RNA to be hybridized to Affymetrix Human PrimeView arrays were processed using the Affymetrix GeneChip 3' IVT Plus kits. Three biological replicates were used for both time points post infection.