Identification of downstream molecules associated with TLR4-dependent inflammatory response in primary bone marrow-derived macrophages (BMDMs) by PolyA-RNA sequencing.

To explore the downstream molecules that are responsible for the regulatory role of macrophage-specific TLR4 during inflammatory responses, we analyzed the transcriptome profile of BMDMs isolated from tlr4f/f and tlr4f/f-lysM-Cre mice with or without LPS stimulation by PolyA RNA sequencing. Overall design: Primary BMDMs of tlr4f/f and tlr4f/f-lysM-Cre mice were stimulated with 1µg/mL of Lipopolysaccharides (LPS) for 6 hours in vitro. Then, three samples of total mRNAs from each group including control-tlr4f/f, control-tlr4f/f-lysM-cre, LPS-tlr4f/f and LPS-tlr4f/f-lysM-cre were subjected for polyA-RNA sequencing to analyze the effect of macrophage-specific TLR4 on the differential gene expression in response to LPS.