Gene expression profiles in control and Hdac3-KD PGCLCs

In mice, primordial germ cells (PGCs), which are the precursors of gametes, are fate-determined from pluripotent epiblasts. The epigenetic regulation of PGC fate determination remains poorly understood. We identify histone deacetylase 3 (HDAC3) as an epigenetic regulator of PGC fate determination by an RNA interference screen for histone modifier genes involved in PGC-like cell (PGCLC) induction in culture. To comprehensively investigate changes in gene expression following Hdac3-KD during PGCLC induction, we carried out RNA sequencing (RNA-seq) analysis to compare the transcriptome of the Hdac3-KD cells with that of the control KD cells 2 days after induction of PGCLCs.