Effect of AICAR, MET or CC treatment on fresh sperm and following cryopreservation.

Sperm were treated with 2mM AICAR, 1mM MET or 5μM CC. The sperm motility parameters including motile sperm (%), rapid sperm (%), progressive (%), path velocity (VAP, in μm/s), progressive velocity (VSL, in μm/s), curvilinear velocity (VCL, in μm/s), straightness coefficient (STR, in %), linearity (LIN, in %) were evaluated by the computer-assisted sperm analysis (CASA) system. Sperm viability (%) was assessed using SYBR-14/PI. Acrosome reaction (AR, %) was assessed using a FITC-PNA staining. Values represent means ± SEM from 6 different experiments (4 samples/experiment).
Different superscripts in lines indicate significant differences between control (Ctrl) and treatments in frozen-thawed semen (a,b,c, P<0.05). Asterisks in lines indicate statistically significant differences between fresh and frozen-thawed semen (*, P<0.05).
Effect of AICAR, MET or CC treatment on fresh sperm and following cryopreservation.