Margaret Clarke, Ulrike Engel, Jennifer Giorgione, Annette Müller-Taubenberger, Jana Prassler, Douwe Veltman, Günther Gerisch (2011) CIL:30546, Dictyostelium discoideum, Saccharomyces cerevisiae, amoeboid cell, budded yeast. CIL. Dataset

Dictyostelium discoideum (ameoba) phagocytosing budded yeast (S. cerevisiae). The amoeba is expressing mRFP-LimEdelta (labels actin filaments, red) and PHcrac-GFP (labels membranes containing phosphatidylinositol (3,4,5)-trisphosphate and phosphatidylinositol (3,4)-bisphosphate, green) Images were collected with a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective. The time series was acquired in a single focal plane at 3.94-second intervals. Pixel size is 0.14 x 0.14 microns.

Dictyostelium discoideum（变形虫）吞噬芽孢酵母（S. cerevisiae）。该变形虫表达mRFP-LimEdelta（标记肌动蛋白纤维，红色）和PHcrac-GFP（标记含有磷脂酰肌醇（3,4,5）-三磷酸和磷脂酰肌醇（3,4）-二磷酸的膜，绿色）。图像采集使用配备63倍1.4数值孔径微分干涉（DIC）物镜的蔡司LSM 510激光扫描共聚焦显微镜。时间序列图像在单焦平面以3.94秒的间隔进行采集。像素大小为0.14 x 0.14微米。