AKT1 AND AKT2 INDUCE DISTINCT PHOSPHORYLATION PATTERNS IN HL-1 CARDIAC MYOCYTES.

Data from ProteomeXchange, PXD ID: PXD000268. Experiment: DML2, file: folder summary. Published as part of J Proteome Res. 2014 Aug 27 . From the Abstract: {{i}} ... To gain more insight into AKT isoform specific signaling at the molecular level we investigated the phosphoproteome of HL-1 cardiomyocytes carrying AKT1 or AKT2 isoform specific knock down, respectively. We combined stable isotope labeling with high resolution mass spectrometry and identified 377 regulated phosphopeptides. Although AKT1 is expressed at four-fold higher levels, insulin stimulation mainly activated AKT2, which might in part rely on a preferred interaction of AKT2 with mammalian target of rapamycin complex 2. In line with this result, the highest number of regulated phosphopeptides was identified in the AKT2 knock down cells. ... {{/i}}