Investigation of differential microRNAs in the exosomes of caerulein-induced AR42J pancreatic acinar cells

We use the illumina high-throughput sequencing technology to identify miRNAs between the exosomes of AR42J cells with or without caerulein-induced.The AR42J cells were cultured in serum-free medium and stimulated with caerulein (10^-7 mmol/L) or PBS for 6 h, then collected the exosomes samples from serum-free medium. Exosomes were isolated and extracted by differential centrifugation and detected by electron microscopy, nanoparticle tracking analysis and exosome surface markers.In total, 30 differentially expressed miRNAs (22 upregulated and 8 downregulated) were screened by miRNA sequence.