Identifying genes directly regulated by Gal1 through its DNA promoter binding in pancreatic stellate cells (PSCs). Identifying genes directly regulated by Gal1 through its DNA promoter binding in pancreatic stellate cells (PSCs)

Galectin-1 (Gal1) is highly expressed in activated PSCs nuclei, a cell type which plays a key role in PDA progression. In order to identify whether Gal-1 is able to recognize regulatory gene elements and regulate their expressión we performed a chromatin immunorecipitation assay followed by sequencing (ChIP-seq). Its analysis revealed that 16% of Gal-1 targets correspond to gene promoters, Indeed, most of these candicates are cancer-associated genes. Among them we found KRAS, which plays an important role in PSC activation. ChIP-seq results were validated by ChIP-qPCR using two independent Gal1 antibodies. These results identify a novel role for nuclear Gal1 by controlling transcriptional gene expression of cancer-associated genes in PSC in the PDA TME. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Galectin-1 in activated pancreatic stellate cells