A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs

EM Quantitation: Raw data gathered from EM images taken to determine nanodisc population size distribution. NNB TGN46 analysis: Data analysis of the Native Nanobleach experiments of TGN46 in native nanodiscs to determine population distribution of oligomeric organizations. Polymer conditions: Physiochemical characteristic and extraction conditions for all polymers in the library both commercially available and in-house. Protein groups polymer screen original file: Original output of MaxQuant data processing of polymer screen data. Organelle matching: Code used for mathcing proteins identified in the proteomics output to organelle or residence for all organellar annotations. Polymer code: Code used to process and normalize the MaxQuant output and calulate extraction efficiency across all detected proteins. MAP Library Details: Graphic and table explaining chemical details of all polymer used in the screen, both commerically available and in-house synthesized. NNB TGN46: Raw scope files for the TIRF microscopy single molecule step photobleaching experiment with TGN46. Organellar Breakdown Database: Proteins detected in the polymer screen through proteomics experiments stratified into organelle of residence. Human Proteome FASTA: The FASTA file used for proteome searching in processing the proteomics data to build the screening database. Hand Curated Organellar Proteomes: Organellar proteomes used for organellar sorting and identification of proteins detected in the screen. Polymer SEC Superdex75: Size exculsion chromatography traces for chloroSMA series of polymers. Was used to characterize length and population polydispersity. Negative Stain Raw: RAW TEM scope images of purified synaptophysin-vamp2 containing nanodiscs. Populatoin size distribution was determined. FSEC Polymer CS80: Fluoresence size exclusion chromatogram for purified synaptophysin-vamp2 containing nanodiscs to ensure population homogeneity and purity. NMR Raw data: NMR raw files for characterizing the in-house synthesized Chloro-SMA series and AASTY series.