Secondary siRNA screen (poly(A)-FISH and RNA StrandBrite)

Image-based genetic screen targeting 463 genes in human HeLa cells using RNA interference (siRNA). Cells stained with DAPI (DNA), poly(A) fluorescence in situ hybridisation (FISH) (mRNA), RNA Strandbrite (total RNA), succinimidyl ester-Alexa647 (total protein). Cells fixed 72h after siRNA transfection. Imaged at 20X magnification with two experimental replicates per condition. Further description available in Berry et al., 2022. Single-cell features provided as background-subtracted mean and sum intensity values for the nucleus, cytoplasm, or whole-cell, as appropriate, for each fluorescence channel. Data cleanup and correction applied as described in the manuscript. Summaries provided on a per-well basis.