High-content phenotypic analysis defines generalizable genetic and molecular regulators of lifespan in a C. elegans recombinant inbred population

Background and Aims: Lifespan is influenced by complex interactions between genetic and environmental factors. Limitations in studying those factors in model organisms of a single genetic background hinder translational value. Here, we mapped genetic determinants of lifespans in 85 C. elegans recombinant intercross advanced inbred lines (RIAILs). We assessed molecular profiles – transcriptome, proteome, and lipidome – and life-history traits (lifespan), development, growth dynamics, and reproduction. Overall design: Total RNA samples were extracted from 85 RIAILs and two parental strains (N2 and CB4856) and subjected to RNA seq.