Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry
收藏NIAID Data Ecosystem2026-05-26 收录
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The purpose of the experiment was to provide comparative data to show quantitatively how improper techniques adversely affect the preparation of a single cell suspension from solid tissues for flow cytometry. Conclusion: Freezing tissue, using a tissue homogenizer, vortexing cells, and over or under digesting tissues when preparing a single cell suspension resulted in decreased cell yield and viability and increased cell debris and fragments when compared to best practice procedures. CS&T Beads (BD Biosciences, San Jose, CA) were used to determine optimal PMT voltages and standardize the settings in FACSDiva 8.0.1.
创建时间：
2018-12-01
