Defining the Phasor Mixing Coefficient

The underlying simulated image data for Figure 3 of <b><i>Redefining colocalization analysis with a novel Phasor Mixing Coefficient.</i></b>The TIFF files A_ch1_af561_spec.tif and A_ch2_af647_spec.tif are artificial multispectral images simulated from intensity image inputs from Figure 1A of the associated manuscript (A_ch1.tiff and A_ch2.tiff, respectively). Their names indicate the real fluorescent spectra from which they are simulated (AlexaFluor(AF)-561 and AF-647, respectively). Ch1 always corresponds to AF561 and Ch2 corresponds to AF647. Spectra are given as CSV files and named accordingly. The TIFF file A_afcomb_spec.tif corresponds to the element-wise sum of the two channel images. The analysis outputs are produced through the "Figure 3" section of the Jupyter notebook in the associated GitHub repository.