Brito_Supplementary Material_revised.docx

<br>Illustrative videos for Supplementary Material<b>Caspase-1 Activation Drives Vascular Inflammatory Processes and Hypoperfusion in Intravascular Hemolysis</b> Legends for videos:<b>Video 1: CONTROL + DMSO. </b>Representative venule from a mouse that<b> </b>was pretreated with DMSO and then received i.v. sterile 0.9% NaCl (at approximately 15-30 min after i.v. administration). <b>Video 2: Acute IVH + DMSO. </b>Representative venule from a mouse that<b> </b>was pretreated with DMSO and then received i.v. sterile water to induce intravascular hemolysis (at approximately 15-30 min after i.v. administration). <b>Video 3: Acute IVH + YVAD. </b>Representative venule from a mouse that<b> </b>was pretreated with YVAD and then received i.v. sterile water to induce intravascular hemolysis (at approximately 15-30 min after i.v. administration). <b>Video 4: CONTROL + PBS. </b>Representative venule from a mouse that<b> </b>was pretreated with PBS and then received i.v. sterile 0.9% NaCl (at approximately 15-30 min after i.v. administration). <b>Video 5: Acute IVH + PBS. </b>Representative venule from a mouse that<b> </b>was pretreated with DMSO and then received i.v. sterile water to induce intravascular hemolysis (at approximately 15-30 min after i.v. administration). <b>Video 6: Acute IVH + MCC950. </b>Representative venule from a mouse that<b> </b>was pretreated with MCC950 and then received i.v. sterile water to induce intravascular hemolysis (at approximately 15-30 min after i.v. administration). <b>Video 7: SCD + Vehicle. </b>Representative venule from a Townes SCD mouse pretreated with sterile 10 mM NaOH vehicle (at approximately 60-80 min after NaOH administration. <b>Video 8: SCD + DMSO + Heme. </b>Representative venule from a Townes SCD mouse that<b> </b>was pretreated with DMSO and then received 32 μM heme (i.v.) in 10 mM NaOH vehicle to induce vaso-occlusive processes (at approximately 60-80 min after heme administration). <b>Video 9: SCD + YVAD + Heme. </b>Representative venule from a Townes SCD mouse that<b> </b>was pretreated with YVAD and then received 32 μM heme (i.v.) in 10 mM NaOH vehicle to induce vaso-occlusive processes (at approximately 60-80 min after heme administration). <br><br><br>