Light sheet imaging of the human brain

This dataset contains images of 1 mm sections of human brain tissue showing nuclei, NeuN+ cells and blood vessels. Each tissue section was first SHIELD-processed for protein protection and delipidated to clear the tissue. The tissues were stained with YOYO1 (nuclei), anti-NeuN antibody (with Rhodamine Red-X secondary antibody) and Lectin (blood vessel) for 8 days in total and then optically cleared using ExPROTOS (a refractive matching solution). The sample was imaged using light sheet microscopy at a resolution of ~2.5 um x 3.6 um x 2.5 um. Each slab was imaged using multiple stacks. Offset transforms are included with the dataset to enable reconstruction of each slab.