RNA sequencing (RNA-seq) for identifing differentially expressed genes for mitochondrial unfolded protein response in Arabidopsis

The purpose of this study is to identify differentially expressed expressed genes for mitochondrial unfolded protein response in Arabidopsis. A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. We using an optimized data analysis workflow, RNA isolated from seedlings of 7-day old wild type arabidopsis plants(Col-0) treated with H2O, Dox and EtBr,and with 3 repeats of each sample