Identification of Pregnancy-specific Transcriptional Mediators of Pancreatic Islet Adaptation Regulated by Prolactin Receptor Signaling in Mice

To identify nodal transcriptional regulators and effector of PRLR signaling gene targets and networks within beta-cells during pregnancy, we compared the transcriptional signatures of islets from βPRLRKO mice relative to those of littermate controls. Gene expression was assessed using Affymetrix GeneChip® Mouse Gene 2.0 ST Array. Target genes were technically validated using real-time quantitative PCR. 6 pancreatic beta islet samples were analyzed by Affymetrix array: 3 wild-type samples; 3 bPRLR-KO samples.