The dataset for the article "Charge-Transfer Engineering By HPHT-O Nanodiamond and Au Nanoparticle Hybrid Architecture for Ultrasensitive Electrochemical Cortisol Immunosensing"

____________________Dataset for "Charge-Transfer Engineering By HPHT-O Nanodiamond and Au Nanoparticle Hybrid Architecture for Ultrasensitive Electrochemical Cortisol Immunosensing"____________________ Last updated: 2026-07-10 ______Authors______* Sagar Ravishankar Maleyur Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 16627 Prague 6, Czechia Role: Data collector * Chakavak Esmaeili Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 16627 Prague 6, Czechia * Chamseddine Madouri Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 16627 Prague 6, Czechia * Anna Artemenko Institute of Physics, Czech Academy of Sciences, Cukrovarnicka 112, 162 00 Prague 6, Czechia * Bohuslav Rezek Faculty of Electrical Engineering, Czech Technical University in Prague, Technicka 2, 16627 Prague 6, Czechia ______Contact______* Egor Ukraintsev* Faculty of Electrical Engineering, Czech Technical University in Prague* Technicka 2, 16627 Prague 6, Czechia ______License______*Dataset for "Charge-Transfer Engineering By HPHT-O Nanodiamond and Au Nanoparticle Hybrid Architecture for Ultrasensitive Electrochemical Cortisol Immunosensing" © 2026 by Egor Ukraintsev is licensed under CC-BY*license information: https://creativecommons.org/licenses/by/4.0/ ------------------------------------------------------------------------------------------------______About the dataset______Reliable electrochemical biosensors require sensing interfaces that simultaneously provide efficient charge transport, stable biofunctionalization, and reproducible analytical performance. Here, we introduce a resistive-conductive nanohybrid interface based on oxidized high-pressure high-temperature nanodiamonds (HPHT-O) and citrate-stabilized gold nanoparticles (C-AuNPs) assembled on gold screen-printed electrodes (AuSPE’s) for cortisol immunosensing. Unlike conventional nanocomposite platforms that primarily exploit conductivity and surface area enhancement, the architecture regulates antibody grafting and electron-transfer pathways through the integration of an electrically insulating, hydrophilic, oxygen-rich nanodiamond scaffold with discrete conductive C-AuNP charge-transfer nodes. Surface and compositional analyses by SEM, FTIR, and XPS confirm formation of the nanohybrid interface and antibody immobilization. Comparative analyses reveal that HPHT-O improves interfacial stability and reproducibility and in synergy with C-AuNPs promotes electron-transfer kinetics. Their combination outperforms single component sensors, exhibiting a linear response from 1 x 10² to 3.625 x 10⁴ pg/mL, limit of detection 14.21 pg/mL by differential pulse voltammetry and 9.41 pg/mL by electrochemical impedance spectroscopy. The hybrid sensors demonstrate high reproducibility, low cross-reactivity toward structurally related steroid hormones, and accurate recovery in artificial saliva. The nanodiamond/C-AuNP-based interfacial charge-transfer engineering thus provides an effective strategy for creating robust electrochemical biosensors for biomarker detection in complex biological matrices. ______Methods of data collection______Electrochemical data (CV, DPV, EIS) were collected with a disposable AuSPE (DRP-220AT-U75, Metrohm DropSens) connected to a portable EmStat4S potentiostat (PalmSens BV) run with PSTrace 5.12. CV: -0.3 V to 0.6 V, 0.01 V step, 0.1 V/s, 3 cycles. DPV: -0.4 V to 0.5 V, 0.01 V step, 0.2 V pulse amplitude, 0.02 s pulse width, 0.05 V/s. EIS: DC 0.2 V, AC 0.01 V, 100 kHz-1 Hz. All measurements used a 3 mM [Fe(CN)6]3-/4- redox probe in 10 mM PBS (pH 7.4). XPS data were acquired with an AXIS Supra spectrometer (Kratos Analytical) with monochromatic Al Ka source (1486.6 eV), survey at 80 eV pass energy, HR spectra at 10 eV, 90 deg take-off angle, 0.3x0.7 mm2 analysis area. ______Methods of data processing______DPV/EIS calibration and interference data were processed to peak current (DPV) and Rct/Cdl via Randles-circuit fitting (EIS); calibration curves fitted as linear regressions of signal vs. log(concentration). XPS HR spectra were peak-fitted in CasaXPS (Shirley background, Gaussian/Lorentzian GL(30)), calibrated on Au 4f at 84.0 eV (bare electrode) or referenced to 285.0 eV (sp3 C, HPHT-O samples). ______Acknowledgments______This research was supported by the MEYS project CZ.02.01.01/00/22_008/0004596 (SenDISo), under the Operational Program Johannes Amos Comenius. Additionally, we acknowledge support from the Czech NanoLab infrastructure (LM2023051) at the Institute of Physics of the Czech Academy of Sciences. This work was carried out with the support of the laboratory of Special Microscopy at the Czech Technical University in Prague. ------------------------------------------------------------------------------------------------ ______File name structure_____* SenDISo_048_CVUT_Y_0000_v1 SenDISo - project prefix 048 - dataset sequence number CVUT - institution abbreviation Y - file type (1 char.): M - manuscript, D - data, S - supplementary information, P - published version of the article 0000 - order of the files in the dataset (4 char.) v1 - versioning (v + version number) ______File formats______* xlsx = raw electrochemical (CV/DPV/EIS) and XPS measurement data, multi-sheet Excel workbooks ------------------------------------------------------------------------------------------------______List of files______* SenDISo_048_CVUT_D_0001_v1.xlsx - CV/DPV/EIS raw data for stepwise AuSPE functionalization, control experiment (single vs. dual nanomaterial), and hybrid-platform calibration/interference/saliva-recovery data (multi-sheet workbook, see sheet names in file) Fig. 1-3 & Fig. S1 source data - stepwise electrode functionalization (CV/DPV/EIS) and control experiment (HPHT-O/C-AuNP hybrid platform) * SenDISo_048_CVUT_D_0002_v1.xlsx - DPV/EIS calibration (DPV_LR, EIS_LR), interference study, and artificial-saliva recovery data for the HPHT-O-only immunosensor platform (multi-sheet workbook) Fig. 4 & Table 2 source data - HPHT-O-only platform (Platform 1) * SenDISo_048_CVUT_D_0003_v1.xlsx - XPS high-resolution C1s/N1s spectra and peak-fit components (atomic concentration and bond concentration) for bare electrode, Electrode_HPHT-O, Electrode_HPHT-O_AuNPs, Electrode_HPHT-O_AuNPs_mAb samples Table S1, Table S2, Fig. S2, Fig. S3 source data - XPS * SenDISo_048_CVUT_S_0004_v1.docx - XPS experimental section, results/discussion text, Table 1 (atomic concentrations) and Table 2 (C1s bond concentrations) as authored by A. Artemenko - source text for Table S1/S2 and Fig. S2/S3 captions XPS methods & results write-up