Data for: Environmental DNA storage and extraction method affects detectability for multiple aquatic invasive species

Environmental DNA (eDNA) refers to genetic material released by organisms into their surrounding environment. Collecting and identifying eDNA has gained popularity for monitoring and surveillance of aquatic invasive species. Invasive species management is most successful when an invasion is identified early while population size is likely to be low, highlighting the importance of eDNA detection sensitivity. Various factors influence DNA yield recovered from environmental samples. Environmental DNA storage and extraction methods, for example, can be adjusted to maximize DNA yield, thereby improving detectability. In this study, we compared the performance of two eDNA storage and extraction methods in detecting three common aquatic invasive species (Bythotrephes longimanus, Dreissena polymorpha, and Faxonius rusticus) across five natural ecosystems of Minnesota, United States. One method involved storing filters in 95% ethanol (EtOH) and extracting DNA using a DNeasy PowerSoil Pro Kit (Qi..., , , # Data for \"Environmental DNA storage and extraction method affects detectability for multiple aquatic invasive species\"

[https://doi.org/10.5061/dryad.r2280gbm8](https://doi.org/10.5061/dryad.r2280gbm8)

## Description of the data and file structure

#### **File name**: storage_extraction_data.csv

**Overview of file:** This file contains data used in statistical tests for the eDNA storage-extraction section of the manuscript.

**Columns**:

“Sample ID”: A unique identifier for each sample.

“Lake”: The lake or water body in which the sample was collected.

“Collection Date”: The date the sample was collected in month/day/year format.

“Point”: One of five sampling points (or locations) within the waterbody labeled as 1, 3, 5, 7, and 10.

“Replicate”:  The numbered (1,2,3) field replicate taken at each sampling point.

“Preservation”: The filter preservation method as ETOH or CTAB.

“Extraction Method”: The DNA extraction method as Qiagen DNeasy Powersoil Pro Kit or phenol-chloroform-...