L1 Retrotransposon sequencing in Cocaine Use Disorder - Study 1

Cocaine Use Disorder (CUD) is a global public health problem, with no effective pharmacotherapies. While CUD is marked by high heritability, discovery of variants associated with risk for CUD has proven difficult. We used Restriction Enzyme Based Enriched L1 sequencing (REBELseq) to amplify L1 retrotransposons in gDNA isolated from medial prefrontal cortex NeuN+ neuronal nuclei from 25 CUD cases and 26 controls. Amplified libraries were sequenced on the Illumina HiSeq 4000 platform, and sequencing data was analyzed with the REBELseq bioinformatics pipeline.]]>
Cocaine Use Disorder: CUD cases were adult individuals who died of acute cocaine toxicity. CUD diagnosis was established postmortem through medical records and family interviews. Controls: Adult individuals who died of non-substance use related cause. Subjects were excluded for causes of death causing acute neuronal damage or prolonged hypoxia (e.g. suicide by gunshot wound to the head or hanging).]]>