Layer-specific proteomic analysis of human hearts in patients with sudden cardiac death

Recent studies have shown that decreased longitudinal strain in speckle-tracking echography can predict sudden cardiac death (SCD) in patients with cardiac hypertrophy (CH). Histologically, the heart wall consists of the inner longitudinal, middle circular, and outer longitudinal layers. Thus, layer-specific proteomic changes may contribute to SCD risk. The three layers in human cardiac tissues were obtained during autopsies of SCD, compensated CH, and control cases (18 cases aged > 40 years, 54 samples in total). SCD cases consisted of patients with ischemic or hypertensive heart failure, whereas CH and control cases were accidental deaths. After histological analysis, cardiomyocytes were collected separately from the three layers of the left ventricular wall using laser microdissection. The extracted proteins were analyzed using liquid chromatography–tandem mass spectrometry, followed by label-free quantification. Histologically, cardiomyocytes were enlarged in all layers of SCD an..., , # Data from: Layer-specific proteomic analysis of human hearts in patients with sudden cardiac death

Dataset DOI: [10.5061/dryad.q573n5tx2](https://doi.org/10.5061/dryad.q573n5tx2)

## Description of the data and file structure

Proteome profiles were individually determined by using a Q Exactive mass spectrometer (Thermo Fisher Scientific) equipped with an UltiMate 3000 LC system (Thermo Fisher Scientific). All patients were divided into three groups: SCD, CH, and Control. All samples were dissected from three myocardial layers: inner longitudinal layer (I), middle circular layer (M), and outer longitudinal layer (O) using laser microdissection (LMD 6500; Leica Microsystems, Wetzlar, Germany)

Note: The mznld files contain raw MS data, while the three CSV files contain processed quantitative protein data extracted from those mznld files. Each CSV file contains protein-level quantification results for one myocardial layer.

## Description of the data and file structure

File name inclu..., Written informed consent for the academic use of the tissue was obtained from the relatives of all decedents included in the study, and the study was approved by the institutional ethics committee (approval number: 21R177). This study was conducted in accordance with the ethical standards of the 1964 Declaration of Helsinki and its subsequent amendments.