小鼠毒性实验原始、分析与检测数据

急性毒性实验：将50只健康ICR小鼠通过随机数字表随机分为5组；每组由10只小鼠（n = 10），雌雄各半组成。根据前期预试验结果确定急性毒性实验的剂量分别为200mg/kg、263mg/kg、346mg/kg、455mg/kg和600mg/kg，使用生理盐水进行配置。禁食过夜后，在小鼠尾静脉通过静脉注射的方式给予各剂量的微纳机器人混悬液。密切观察14天内死亡或行为变化和毒性临床症状，例如皮肤、流泪、进食和睡眠的变化。根据改良寇氏法计算半数致死剂量（LD50）和最大耐受剂量（MTD）。亚急性毒性实验：健康的ICR共48只，雌性与雄性小鼠各半，共分为4组，每组12只（n=12），包括3个实验组和1个生理盐水对照组。对照组予尾静脉注射生理盐水，实验组根据急性毒性实验结果经尾静脉注射低剂量（4.97 mg/kg）、中剂量（19.87 mg/kg）和高剂量（79.48 mg/kg）的微纳机器人混悬液，每周5次，共28d。每天密切观察所有动物是否有死亡或是否存在行为变化和中毒症状。并测量小鼠的体重。在28天的研究结束时，用1%戊巴比妥钠麻醉小鼠。通过摘眼球取血样，将血样收集在抗凝管1.5mlEP管中用于全血血液学测定，收集在不抗凝1.5mlEP管中进行肝肾等检测。采血后，脱颈处死，并收集脑、肺、心、肝、肾、脾、卵巢和睾丸。使用数字天平立即测量湿器官重量。使用公式：相对器官重量 (%) =器官重量 (g)/身体重量 (g) x 100%进行计算。随后将所有器官组织固定在4%多聚甲醛中，经充分固定后，脱水，包埋，切片，并用苏木精-伊红染料、普鲁士蓝染色，显微镜拍照观察。

Acute Toxicity Test: Fifty healthy ICR mice were randomly assigned to 5 groups via a random number table. Each group comprised 10 mice (n = 10), with an equal distribution of males and females. The doses for the acute toxicity test were determined as 200 mg/kg, 263 mg/kg, 346 mg/kg, 455 mg/kg, and 600 mg/kg based on the results of a preliminary trial, and were prepared using normal saline. After being fasted overnight, each group was administered the corresponding dose of micro-nanorobot suspension via tail vein intravenous injection. Mortality, behavioral changes, and clinical toxic symptoms (such as changes in skin appearance, lacrimation, feeding behavior, and sleep patterns) were closely monitored over a 14-day observation period. The median lethal dose (LD50) and maximum tolerated dose (MTD) were calculated using the modified Karber's method.

Subacute Toxicity Test: A total of 48 healthy ICR mice, with equal numbers of males and females, were divided into 4 groups, with 12 mice per group (n = 12), including 3 experimental groups and 1 normal saline control group. The control group was injected with normal saline via the tail vein, while the experimental groups were administered micro-nanorobot suspensions at low (4.97 mg/kg), medium (19.87 mg/kg), and high (79.48 mg/kg) doses via tail vein injection based on the results of the acute toxicity test. The administration was performed 5 times per week for a total of 28 consecutive days. All animals were closely monitored daily for mortality, behavioral changes, and signs of intoxication. The body weight of each mouse was measured daily. At the end of the 28-day study, the mice were anesthetized with 1% sodium pentobarbital solution. Blood samples were collected via eyeball extirpation: samples collected in 1.5 mL anticoagulant-treated EP tubes were used for complete blood count (CBC) analysis, while those collected in 1.5 mL non-anticoagulant EP tubes were used for hepatic and renal function assays. Following blood collection, the mice were euthanized via cervical dislocation, and organs including the brain, lung, heart, liver, kidney, spleen, ovary, and testis were harvested. Wet organ weights were measured immediately using a digital analytical balance. The relative organ weight (%) was calculated using the formula: relative organ weight (%) = (organ weight (g) / body weight (g)) × 100%. All organ tissues were then fixed in 4% paraformaldehyde solution. After sufficient fixation, the tissues were dehydrated, embedded, sectioned, stained with hematoxylin-eosin (H&E) and Prussian blue, and finally observed and photographed under a microscope.

搜集汇总

背景与挑战

背景概述

该数据集包含小鼠毒性实验的原始、分析与检测数据，聚焦于评估微纳机器人在ICR小鼠中的急性毒性和亚急性毒性效应。实验设计包括剂量分组、静脉注射、临床观察、体重测量、器官重量计算和组织学分析，数据量246.01MB，共69个文件，由中日友好医院发布，支持药物输送技术的安全性研究。