RNA sequencing analysis of unilateral orchidectomy, bilateral orchidectomy, and Nell2 knockout initial segment-caput epididymis.

Purpose: The purpose of this study is to characterize gene expression in the Ovch2-ablated mouse initial segment (IS)-caput epididymis. Methods: Ovch2-/- mice were used when they were 14-week-old. IS-caput epididymal mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes was not critically affected by Ovch2 ablation. IS-caput epididymal mRNA profiles of mice with experimental treatments were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000.研究目的：本研究旨在表征Ovch2基因敲除小鼠附睾初始节（IS）-附睾头段的基因表达特征。 实验方法：选用14周龄的Ovch2纯合敲除（Ovch2-/-）小鼠，采用Illumina NovaSeq6000测序平台，通过三次生物学重复进行深度测序，获取该组织的mRNA表达谱。 实验结果：RNA测序数据成功鉴定出差异表达转录本。 研究结论：本研究结果显示，多数基因的表达未受Ovch2敲除的显著影响。本研究同样采用Illumina NovaSeq6000测序平台，通过三次生物学重复完成深度测序，获取了经实验处理小鼠的附睾初始节-附睾头段mRNA表达谱。