Cystic fibrosis (CF) is the most common lethal genetic disease, caused by CFTR (cystic fibrosis transmembrane conductance regulator) gene mutations. CF is characterized by an ionic imbalance and thickened mucus, leading to impaired mucociliary clearance and promoting bacterial colonization and the establishment of infection/inflammation cycles. However, the origin of this inflammation remains unclear. The goal of this study was to investigate the differential expression of mRNA in CF patients to determine new therapeutic strategy.

Primary Bronhcial epithalial cells from CF and non-CF patients were cultured in resting condition in air liquid interphace.