price.idSC.filtered.raw.counts.v1.csv
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https://figshare.com/articles/dataset/price_idSC_filtered_raw_counts_v1_csv/25917505
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资源简介：
High-throughput 3' DGE library preparation was conducted as per:
dx.doi.org/10.17504/protocols.io.bumynu7w
with 6 nucleotide barcodes on R2 for the 384 wells.
Reads were demultiplexed using a custom script and aligned to mm10 via bwa aln -l 24117.
Gene expression levels were determined by counting unique UMIs among reads with unambiguous gene mappings.
The resulting expression matrix was further filtered by removing:
(1) samples with fewer than 3000 genes detected;
(2) RNA associated genes ("Mt_tRNA", "Mt_rRNA", "snoRNA", "snRNA", "misc_RNA", "scaRNA", "rRNA", "sRNA");
(3) genes with fewer than 10 counts total that are seen in less than 4 samples.
创建时间：
2024-06-28
