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TNF response in promyelocytic and granulocytic forms of HL60/S4 cells

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120579
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The human promyelocytic cell line HL60/S4 can be differentiated into a granulocytic form with the addition of all-trans retinoic acid for 4 days. We investigated the conserved and differentiated responses to TNF in the granulocytic and promyelocytic forms of HL60/S4 cells. Promyeloytic and granulocytic HL60/S4 cells were treated with 16ng/ml TNF or equivalent volume of vehicle (water) in calcium supplemented growth media for two hours, then RNA was extracted with Trizol LS and the Qiagen Rneasy mini kit.
创建时间:
2019-08-21
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